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rad18 cell signaling technology  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rad18 cell signaling technology
    Rad18 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rad18+cell+signaling+9040/Rad18+XP+Rabbit+mAb/pmc11909274__41467_2025_56981_MOESM2_ESM-27-82-83
    Average 94 stars, based on 57 article reviews
    rad18 cell signaling technology - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single-stranded DNA gaps.
    Article Snippet: on 08 D ecem ber 2024 owing oligonucleotide sequences (Stealth or SilencerSelect iRNA, Thermo Fisher Scientific) were used: PRIMPOL: ID: 39536; HELQ#1: C AC AGAGAACC AGAGUGGAU AUGAA; HELQ#2: ID:125860; HELQ#3: ID: s41483; NBN: ID: s529215; PARP2: ID: s19504; POLD3#1: ID: s21045; POLD3#2: ID: 119736; RAD52#1: ID: s11746; RAD52#2: ID: s532174; MUS81: UUUGCUGGGUCUCUA GGA UUGGUCU; ZRANB3: UGGC AAUGU AGUCUCUGC ACCU AU A; UBC13: ID: s14595 RAD18: ID: s32295 Denatured whole cell extracts were prepared by boiling ells in 100 mM Tris, 4% sodium dodecyl sulfate and 0.5 M βercaptoethanol

    Western Blot:

    Article Title: CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single-stranded DNA gaps
    Article Snippet: The following oligonucleotide sequences (Stealth or SilencerSelect siRNA, Thermo Fisher Scientific) were used: PRIMPOL: ID: 39536; HELQ#1: CACAGAGAACCAGAGUGGAUAUGAA; HELQ#2: ID:125860; HELQ#3: ID: s41483; NBN: ID: s529215; PARP2: ID: s19504; POLD3#1: ID: s21045; POLD3#2: ID: 119736; RAD52#1: ID: s11746; RAD52#2: ID: s532174; MUS81: UUUGCUGGGUCUCUAGGAUUGGUCU; ZRANB3: UGGCAAUGUAGUCUCUGCACCUAUA; UBC13: ID: s14595 RAD18: ID: s32295 Denatured whole cell extracts were prepared by boiling cells in 100 mM Tris, 4% sodium dodecyl sulfate and 0.5 M β-mercaptoethanol. .. Antibodies used for western blot, at 1:500 dilution, were: PRIMPOL: Proteintech 29824–1-AP; HELQ: Invitrogen PA5-88692; POLD3: Invitrogen PA5-96618; RAD52: Santa Cruz sc-365341; MUS81: Santa Cruz sc-47692; BRCA2: Bethyl A303-434A; ZRANB3: Invitrogen PA5-65143; UBC13: Santa Cruz Biotechnology sc-376470; RAD18: Cell Signaling 9040; GAPDH: Santa Cruz Biotechnology sc-47724; Vinculin: Santa Cruz Biotechnology sc-73614. .. Inhibitors used were: RAD52 inhibitor – Epigallocatechin (EGC; Sigma–Aldrich, 970–74–1).



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